The metabolism of glucose is linked to diabetes, heart disease and cancer. Our in vivo profiling services such as Glucose Metabolism Service is equipped to measure glucose metabolism measured in mice and rats under minimum stress. The results obtained provide information about mechanism of action, drug safety, drug validation and possible further research and development with clinical implications. The Biology Service provides a wide variety of services for analyzing metabolism in animal models, isolated tissue and tissue culture with support for preclinical development towards candidate selection.

We offer services in the following areas:

• Oral glucose tolerance test
  Study the effect of a drug on glucose disposal and glucose production under conditions that simulate the fed state.
• Insulin tolerance test
  Determines the effect of a drug on insulin-mediated glucose disposal and glucose production.
• Hormone and cytokine assays
  Circulating markers of diabetes and inflammation.
• Signal Transduction
  Identification of cell signaling pathways in tissues of interest by western blot analysis.
• HPLC, GCMS and LC MS/MS
  Identification of parent compound and circulating metabolites.

For the in vivo evaluation of drug substances that modulate cancer pathways, we offer a wide range of cell lines and xenograft rodent models to study the effects of drug substance in a wide variety of cancers such as breast, prostate, colon, peripheral blood, lung, ovarian, thyroid, pancreas, skin, liver and kidney.

We offer services in the following areas:

• Orthotopic Xenograft Model
  Study the effect of drug substance on human tumors transplanted to the equivalent mouse organ along with effects of the drug on preventing metastasis of the tumor under consideration.
• Subcutaneous Xenograft Model
  Study the effect of drug substance on human tumors transplanted under the subcutaneous tissue of mice.
• Model established from tumor fragment
  Establish the mutation responsible for the human tumor and generate corresponding mouse model.
• The following established cell lines and Xenograft models are available:
  - Breast: MCF-7, MX-1, MDA-MB-231, BT-474.
  - Non-small cell Lung: H-460, A-549.
  - Colon: HT-29, COLO-205, HCT-116, SW-620.
  - Prostate: PC-3, DU-145.
  - Skin Melanoma: WM-266-4, A-375, COLO-829.
  - Hepatocellular: HepG2, Hep 3B2.1-7.
  - Kidney: A498, 786, Cakil.
  
• Signal Transduction
  Identification of cell signaling pathways in tissues of interest by western blot analysis.
• HPLC, GCMS and LC MS/MS
  Identification of parent compound and circulating metabolites.

For the in vivo evaluation of drug substances that modulate inflammatory pathways, we offer a wide range of rodent models. We offer to do time as well as dose-response studies to study the hematological and biochemical parameters in order to identify the main stages of the disease and the effect of drug substance of interest.

• Carrageenan Paw Edema Model
  Study the effect on paw diameter, when drug substance is administered before carrageenan injection in Lewis rats in single as well as multiple dose studies.
• Collagen Induced Arthritis Model
  Study the effect on ankle diameter, when drug substance is administered to rats sensitized to collagen.
• Adjuvant Induced Arthritis Model
  Study the effect on ankle diameter, when drug substance is administered to rats given lipoidal in Freunds Complete Adjuvant.
• LPS induced inflammatory Model
  Determine the effect of drug substance on LPS induced inflammatory markers in Lewis rats as well as on metabolic strains of mice.
• Hormone and cytokine assays
  Circulating markers of inflammation.
• Signal Transduction
  Identification of cell signaling pathways in tissues of interest by western blot analysis.
• HPLC, GCMS and LC MS/MS
  Identification of parent compound and circulating metabolites.